Thesis Tiffany GARIN (2020 - 2023)

Impact of interbacterial competition through type VI secretion systems (T6SS) on the dynamics of the seed microbiota

To exploit beneficial seed-microbiota combinations in agriculture, it is necessary to understand the assembly and dynamics of these microbiotas during the early stages of plant development. The habitat offered by seeds is quite small in spatial and resource terms, which induces selection and a major bottleneck for bacterial populations.

As a result, the diversity of the seed microbiota tends to be limited, with few dominant taxons when compared with other habitats in the plant. However, considerable diversity is maintained in the microbial assemblies of seeds. The composition and functions of these assemblies can partly be explained by their diversified metabolic capacities. The compositions of microbiotas can also be regulated by interbacterial interactions by means of specific antimicrobial weapons. Some dominant bacterial phyla in seeds are endowed with such weapons, such as type VI secretion systems (orT6SS) that target other micro-organisms with antimicrobial effectors. At present, the contributions of such secretion systems to the assembly of the seed microbiota, and implicitly to seed performance, are not known. This project aims to explore the role of bacterial T6SS in the dynamics of microbiota assembly in brassica seeds and their direct or indirect impact on the transmission of pathogenic agents by seeds.

Contact - Coordination :

  • Tiffany GARIN (Doctoral Candidate)
  • Alain SARNIGUET (Doctoral Adviser, IRHS, Département SPE)
  • Matthieu BARRET (Doctoral Adviser, IRHS, Département SPE)

Funding :

  • 50% INRAE
  • 50% Région Pays-de-la-Loire

See also

Publication

Tiffany Garin (2024) Impact of inter-microbial competitions mediated by the type VI secretion system on the dynamics of seed microbiota transmission to seedlings
 PhD thesis, Université d'Angers. →  https://theses.fr/2024ANGE0008

Download the thesis file